Prepro-alpha-factor has a cleavable signal sequence.
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چکیده
منابع مشابه
Full-length prepro-alpha-factor can be translocated across the mammalian microsomal membrane only if translation has not terminated
We have previously shown that fully synthesized prepro-alpha-factor (pp alpha F), the precursor for the yeast pheromone alpha-factor, can be translocated posttranslationally across yeast rough microsomal (RM) membranes from a soluble, ribosome-free pool. We show here that this is not the case for translocation of pp alpha F across mammalian RM. Rather we found that a small amount of translocati...
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Posttranslational translocation of prepro-alpha-factor (ppalphaF) across the yeast endoplasmic reticulum membrane begins with the binding of the signal sequence to the Sec complex, a membrane component consisting of the trimeric Sec61p complex and the tetrameric Sec62p/63p complex. We show by photo-cross-linking that the signal sequence is bound directly to a site where it contacts simultaneous...
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We have previously shown that fully synthesized prepro-ct-factor (ppaF), the precursor for the yeast pheromone a-factor, can be transiocated posttranslationally across yeast rough microsomai (RM) membranes from a soluble, ribosome-free pool. We show here that this is not the case for translocation of ppctF across mammalian RM. Rather we found that a small amount of translocation of full-length ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)68773-3